A2231
 |  |  |
 | Immunoblotting Cell lysates of HEK-293T transfected with Mouse Axin1-FLAG expression vector, were separated on SDS-PAGE and blotted with Anti-Axin1 (C-terminal region) (Cat. No. A2231). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1: Antibody dilution 1:5,000 2: Antibody dilution 1:5,000 +Inhibition with Axin1 immunizing peptide (human 815-833) (10 μg/mL) 3: Untransfected HEK-293T cells. Antibody dilution 1:5,000 |
Image
Useful Links & Tools
- MSDS
- Certificate of Analysis
- A2231 - Datasheet (82 KB)
- Bulk Quote
- Similar Products
A2231
Sigma
Anti-Axin1 (C-terminal region) antibody produced in rabbit
~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Axin, Anti-Axis inhibition protein 1, Anti-Axis Inhibitor 1 |
Description
| Immunogen | synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2. |
| Physical form | Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~130 kDa |
| species reactivity | mouse, rat, human |
| concentration | ~2.5 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1 |
| Gene Information | human ... AXIN1(8312) |
| rat ... Axin1(79257) | |
| mouse ... Axin1(12005) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
